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Unveiling the Significance of Same-Day PBMC Processing

  • 2 mins

In the world of biomedical research, the pursuit of accurate and reliable data is never-ending. One crucial factor that significantly impacts the quality of research involving Peripheral Blood Mononuclear Cells (PBMCs) is the timing of processing. Same-day PBMC processing has emerged as a vital practice with wide-ranging implications. In this blog post, we explore the importance of same-day PBMC processing, drawing insights from recent scientific literature that highlight its relevance in improving research outcomes.

Understanding PBMCs:

PBMCs are a diverse group of immune cells that play a crucial role in the body's defense mechanisms. These cells are central to immune responses, making them invaluable in various studies, including immunology, infectious diseases, and cancer research. However, PBMCs are sensitive and can undergo changes during storage or delayed processing.

Preserving Cellular Integrity:

Recent studies emphasize the critical importance of preserving the cellular integrity of PBMCs as close to the time of blood collection as possible. Delayed processing can lead to changes in gene expression, cytokine production, and surface marker expression. This highlights the need for same-day PBMC processing to maintain the cells' physiological state and ensure the accuracy of downstream analyses.

Impact on Immune Profiling:

Accurate immune profiling is crucial in immunological studies. A study published in the Journal of Immunological Methods (2019) highlights that same-day PBMC processing minimizes variations in immune cell subsets and gene expression, enhancing the reliability of immune profiling data. Timely processing ensures that immune phenotypes and functional profiles remain true representations of the in vivo state.

Cytokine Secretion Dynamics:

Cytokines play a vital role in immune responses and provide insights into various diseases. Research in the Journal of Immunology (2020) indicates that same-day PBMC processing is essential for accurately assessing cytokine secretion profiles. Delayed processing can result in cytokine degradation and skewed results, compromising the validity of findings related to cytokines.

Implications for Clinical Studies:

In the realm of clinical research, the significance of same-day PBMC processing is even more pronounced. Studies published in the Journal of Clinical Immunology (2021) emphasize that delayed processing can introduce confounding variables in clinical trial outcomes. Same-day processing ensures that therapeutic responses and biomarker evaluations accurately reflect the patient's condition.


The importance of same-day PBMC processing resonates throughout the scientific community. Recent literature unequivocally underscores the significance of timely processing in maintaining cellular integrity, ensuring accurate immune profiling, understanding cytokine secretion dynamics, and validating clinical research. Researchers seeking robust and reliable results must prioritize same-day PBMC processing to unlock the full potential of these crucial immune cells.

At Avrok, we understand the critical role of same-day PBMC processing in advancing research. With our state-of-the-art facilities and commitment to best practices, we ensure the preservation of cellular integrity, leading to meaningful research outcomes. Contact us today to learn more about how our services can enhance your research endeavors.

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